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lipofectamine 2000 mediated transfection  (Addgene inc)


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    Addgene inc lipofectamine 2000 mediated transfection
    Lipofectamine 2000 Mediated Transfection, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipofectamine+2000+mediated+transfection/pLKO%2E1+sh+OPN-7+(Plasmid+%2320008)/pmc11932930-233-20-16
    Average 93 stars, based on 5 article reviews
    lipofectamine 2000 mediated transfection - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Construct:

    Article Title: A TBX2-driven signaling switch from androgen receptor to glucocorticoid receptor confers therapeutic resistance in prostate cancer
    Article Snippet: The custom constructs for sh-TBX2 and its non-targeting scrambled RNA duplex siRNA control (NTSCR) were procured from Sigma Aldrich (RNAi single clones, Millipore sigma, Burlington, MA). .. These lentiviral constructs along with packaging plasmid (pCMV delta R8.2 dvpr, Addgene#8455) and envelope plasmid (pCMV-VSV-G, Addgene#8454) were used for lipofectamine 2000-mediated transfection of approximately 0.8 × 10 6 293FT cells to produce lentiviral pseudo typed particles. ..

    Plasmid Preparation:

    Article Title: A TBX2-driven signaling switch from androgen receptor to glucocorticoid receptor confers therapeutic resistance in prostate cancer
    Article Snippet: The custom constructs for sh-TBX2 and its non-targeting scrambled RNA duplex siRNA control (NTSCR) were procured from Sigma Aldrich (RNAi single clones, Millipore sigma, Burlington, MA). .. These lentiviral constructs along with packaging plasmid (pCMV delta R8.2 dvpr, Addgene#8455) and envelope plasmid (pCMV-VSV-G, Addgene#8454) were used for lipofectamine 2000-mediated transfection of approximately 0.8 × 10 6 293FT cells to produce lentiviral pseudo typed particles. ..

    Transfection:

    Article Title: A TBX2-driven signaling switch from androgen receptor to glucocorticoid receptor confers therapeutic resistance in prostate cancer
    Article Snippet: The custom constructs for sh-TBX2 and its non-targeting scrambled RNA duplex siRNA control (NTSCR) were procured from Sigma Aldrich (RNAi single clones, Millipore sigma, Burlington, MA). .. These lentiviral constructs along with packaging plasmid (pCMV delta R8.2 dvpr, Addgene#8455) and envelope plasmid (pCMV-VSV-G, Addgene#8454) were used for lipofectamine 2000-mediated transfection of approximately 0.8 × 10 6 293FT cells to produce lentiviral pseudo typed particles. ..



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    ( A ) Schematic representation of wild type, nuclear import (GNL3L ΔNLS ) and nuclear export (GNL3L ΔNES ) defective mutants of GNL3L. ( B ) HeLa cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS expression plasmids and the subcellular localization was analyzed using confocal microscopy. The scale bar represents 20μm. ( C ) HEK293 cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS and the expression was determined by western blot analysis using anti-Flag antibody. (D) Ectopic expression of GNL3L results in decreased accumulation of ‘G2/M’ population. GNL3L WT was overexpressed in HEK293 cells and the asynchronous cell cycle pattern was analyzed using flow cytometry as described in Materials and Methods. (E) Western blot was performed to analyze the protein levels of endogenous cyclins A2 and B1 upon GNL3L WT expression in HEK293 cells. Beta actin served as loading control. (F) <t>GNL3L</t> <t>knockdown</t> leads to increased accumulation of cells in G2/M phase of the cell cycle. Transient knockdown of GNL3L was performed in HEK293 cells using specific <t>siRNA</t> and the cell cycle profile was analyzed using flow cytometry. (NC: Negative control). (G) Endogenous cyclins A2 and B1 levels were analyzed using western blot upon GNL3L knockdown in HEK293.
    Lipofectamine 2000 Mediated Transfection Of Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lipofectamine 2000 mediated transfection  (Addgene inc)
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    ( A ) Schematic representation of wild type, nuclear import (GNL3L ΔNLS ) and nuclear export (GNL3L ΔNES ) defective mutants of GNL3L. ( B ) HeLa cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS expression plasmids and the subcellular localization was analyzed using confocal microscopy. The scale bar represents 20μm. ( C ) HEK293 cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS and the expression was determined by western blot analysis using anti-Flag antibody. (D) Ectopic expression of GNL3L results in decreased accumulation of ‘G2/M’ population. GNL3L WT was overexpressed in HEK293 cells and the asynchronous cell cycle pattern was analyzed using flow cytometry as described in Materials and Methods. (E) Western blot was performed to analyze the protein levels of endogenous cyclins A2 and B1 upon GNL3L WT expression in HEK293 cells. Beta actin served as loading control. (F) <t>GNL3L</t> <t>knockdown</t> leads to increased accumulation of cells in G2/M phase of the cell cycle. Transient knockdown of GNL3L was performed in HEK293 cells using specific <t>siRNA</t> and the cell cycle profile was analyzed using flow cytometry. (NC: Negative control). (G) Endogenous cyclins A2 and B1 levels were analyzed using western blot upon GNL3L knockdown in HEK293.
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    ( A ) Schematic representation of wild type, nuclear import (GNL3L ΔNLS ) and nuclear export (GNL3L ΔNES ) defective mutants of GNL3L. ( B ) HeLa cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS expression plasmids and the subcellular localization was analyzed using confocal microscopy. The scale bar represents 20μm. ( C ) HEK293 cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS and the expression was determined by western blot analysis using anti-Flag antibody. (D) Ectopic expression of GNL3L results in decreased accumulation of ‘G2/M’ population. GNL3L WT was overexpressed in HEK293 cells and the asynchronous cell cycle pattern was analyzed using flow cytometry as described in Materials and Methods. (E) Western blot was performed to analyze the protein levels of endogenous cyclins A2 and B1 upon GNL3L WT expression in HEK293 cells. Beta actin served as loading control. (F) <t>GNL3L</t> <t>knockdown</t> leads to increased accumulation of cells in G2/M phase of the cell cycle. Transient knockdown of GNL3L was performed in HEK293 cells using specific <t>siRNA</t> and the cell cycle profile was analyzed using flow cytometry. (NC: Negative control). (G) Endogenous cyclins A2 and B1 levels were analyzed using western blot upon GNL3L knockdown in HEK293.
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    lipofectamine 2000-mediated transfection protocol  (Thermo Fisher)
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    Thermo Fisher lipofectamine 2000-mediated transfection protocol
    ( A ) Schematic representation of wild type, nuclear import (GNL3L ΔNLS ) and nuclear export (GNL3L ΔNES ) defective mutants of GNL3L. ( B ) HeLa cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS expression plasmids and the subcellular localization was analyzed using confocal microscopy. The scale bar represents 20μm. ( C ) HEK293 cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS and the expression was determined by western blot analysis using anti-Flag antibody. (D) Ectopic expression of GNL3L results in decreased accumulation of ‘G2/M’ population. GNL3L WT was overexpressed in HEK293 cells and the asynchronous cell cycle pattern was analyzed using flow cytometry as described in Materials and Methods. (E) Western blot was performed to analyze the protein levels of endogenous cyclins A2 and B1 upon GNL3L WT expression in HEK293 cells. Beta actin served as loading control. (F) <t>GNL3L</t> <t>knockdown</t> leads to increased accumulation of cells in G2/M phase of the cell cycle. Transient knockdown of GNL3L was performed in HEK293 cells using specific <t>siRNA</t> and the cell cycle profile was analyzed using flow cytometry. (NC: Negative control). (G) Endogenous cyclins A2 and B1 levels were analyzed using western blot upon GNL3L knockdown in HEK293.
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    Image Search Results


    ( A ) Schematic representation of wild type, nuclear import (GNL3L ΔNLS ) and nuclear export (GNL3L ΔNES ) defective mutants of GNL3L. ( B ) HeLa cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS expression plasmids and the subcellular localization was analyzed using confocal microscopy. The scale bar represents 20μm. ( C ) HEK293 cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS and the expression was determined by western blot analysis using anti-Flag antibody. (D) Ectopic expression of GNL3L results in decreased accumulation of ‘G2/M’ population. GNL3L WT was overexpressed in HEK293 cells and the asynchronous cell cycle pattern was analyzed using flow cytometry as described in Materials and Methods. (E) Western blot was performed to analyze the protein levels of endogenous cyclins A2 and B1 upon GNL3L WT expression in HEK293 cells. Beta actin served as loading control. (F) GNL3L knockdown leads to increased accumulation of cells in G2/M phase of the cell cycle. Transient knockdown of GNL3L was performed in HEK293 cells using specific siRNA and the cell cycle profile was analyzed using flow cytometry. (NC: Negative control). (G) Endogenous cyclins A2 and B1 levels were analyzed using western blot upon GNL3L knockdown in HEK293.

    Journal: PLoS ONE

    Article Title: GNL3L Is a Nucleo-Cytoplasmic Shuttling Protein: Role in Cell Cycle Regulation

    doi: 10.1371/journal.pone.0135845

    Figure Lengend Snippet: ( A ) Schematic representation of wild type, nuclear import (GNL3L ΔNLS ) and nuclear export (GNL3L ΔNES ) defective mutants of GNL3L. ( B ) HeLa cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS expression plasmids and the subcellular localization was analyzed using confocal microscopy. The scale bar represents 20μm. ( C ) HEK293 cells were transfected with Flag-tagged GNL3L WT , GNL3L ΔNES and GNL3L ΔNLS and the expression was determined by western blot analysis using anti-Flag antibody. (D) Ectopic expression of GNL3L results in decreased accumulation of ‘G2/M’ population. GNL3L WT was overexpressed in HEK293 cells and the asynchronous cell cycle pattern was analyzed using flow cytometry as described in Materials and Methods. (E) Western blot was performed to analyze the protein levels of endogenous cyclins A2 and B1 upon GNL3L WT expression in HEK293 cells. Beta actin served as loading control. (F) GNL3L knockdown leads to increased accumulation of cells in G2/M phase of the cell cycle. Transient knockdown of GNL3L was performed in HEK293 cells using specific siRNA and the cell cycle profile was analyzed using flow cytometry. (NC: Negative control). (G) Endogenous cyclins A2 and B1 levels were analyzed using western blot upon GNL3L knockdown in HEK293.

    Article Snippet: Transient knockdown of GNL3L was performed by lipofectamine 2000-mediated transfection of siRNA in HEK293 cells (L-015743-01-0005, ON-TARGET plus SMARTpool, Human GNL3L (54552), Thermo Fisher Scientific Inc., USA).

    Techniques: Transfection, Expressing, Confocal Microscopy, Western Blot, Flow Cytometry, Control, Knockdown, Negative Control